Stanley Fields is an American biologist best known for developing the yeast two hybrid method for identifying protein–protein interactions.[1] He is currently a professor of Genome Sciences at the University of Washington and Howard Hughes Medical Institute Investigator,[2][3] and previously served as chair of the Department of Genome Sciences.[4]
Stan Fields | |
|---|---|
| Born | Stanley Fields |
| Alma mater | University of Cambridge |
| Known for | Two-hybrid screening |
| Scientific career | |
| Institutions | |
| Thesis | Sequence analysis of influenza virus RNA (1981) |
| Website | |
Education
editFields was educated at the University of Cambridge where he was awarded a Doctor of Philosophy in 1981 for research carried out in the Laboratory of Molecular Biology with Greg Winter and George Brownlee.[5][6]
Research
editFields developed the yeast two-hybrid system in 1989,[1] which has been widely used by Fields[7][8][9][10] and others to identify protein-protein interactions in various organisms and biological contexts.
Along with Matt Kaeberlein and Brian Kennedy, in later work Fields has carried out genome-wide screens for aging genes in yeast. Kaeberlein and co-workers have questioned the hypothesis that lifespan extension from caloric restriction is mediated by Sirtuins.[11] Instead Kaeberlein, Fields, and Kennedy have proposed that caloric restriction increases lifespan by decreasing the activity of the Target of Rapamycin (TOR) kinase.[12]
Honors and awards
edit- 2003 Jacob Heskel Gabbay Award in Biotechnology and Medicine (jointly with Roger Brent) [13]
References
edit- ^ a b Fields, S.; Song, O. (1989). "A novel genetic system to detect protein-protein interactions". Nature. 340 (6230): 245–246. Bibcode:1989Natur.340..245F. doi:10.1038/340245a0. PMID 2547163. S2CID 4320733.
- ^ Fields, Stanley (2014). "Would Fred Sanger Get Funded Today?". Genetics. 197 (2): 435–439. doi:10.1534/genetics.114.165134. PMC 4063904. PMID 24939989.
- ^ Stanley Fields's publications indexed by the Scopus bibliographic database. (subscription required)
- ^ "UW Genome Sciences: Stanley Fields". www.gs.washington.edu. Retrieved 2023-08-14.
- ^ Winter, G.; Fields, S.; Brownlee, G. G. (1981). "Nucleotide sequence of the haemagglutinin gene of a human influenza virus H1 subtype". Nature. 292 (5818): 72–75. Bibcode:1981Natur.292...72W. doi:10.1038/292072a0. PMID 7278968. S2CID 4312205.
- ^ Fields, S.; Winter, G.; Brownlee, G. G. (1981). "Structure of the neuraminidase gene in human influenza virus A/PR/8/34". Nature. 290 (5803): 213–217. Bibcode:1981Natur.290..213F. doi:10.1038/290213a0. PMID 7010182. S2CID 8051512.
- ^ Bartel, P. L.; Roecklein, J. A.; SenGupta, D.; Fields, S. (January 1996). "A protein linkage map of Escherichia coli bacteriophage T7". Nature Genetics. 12 (1): 72–77. doi:10.1038/ng0196-72. ISSN 1061-4036. PMID 8528255.
- ^ Iwabuchi, K.; Li, B.; Bartel, P.; Fields, S. (June 1993). "Use of the two-hybrid system to identify the domain of p53 involved in oligomerization". Oncogene. 8 (6): 1693–1696. ISSN 0950-9232. PMID 8502489.
- ^ Schwikowski, B.; Uetz, P.; Fields, S. (December 2000). "A network of protein-protein interactions in yeast". Nature Biotechnology. 18 (12): 1257–1261. doi:10.1038/82360. ISSN 1087-0156. PMID 11101803. S2CID 3009359.
- ^ Iwabuchi, K.; Bartel, P. L.; Li, B.; Marraccino, R.; Fields, S. (1994-06-21). "Two cellular proteins that bind to wild-type but not mutant p53". Proceedings of the National Academy of Sciences of the United States of America. 91 (13): 6098–6102. Bibcode:1994PNAS...91.6098I. doi:10.1073/pnas.91.13.6098. ISSN 0027-8424. PMC 44145. PMID 8016121.
- ^ Kaeberlein, M.; Kirkland, K. T.; Fields, S.; Kennedy, B. K. (2004). "Sir2-Independent Life Span Extension by Calorie Restriction in Yeast". PLOS Biology. 2 (9): e296. doi:10.1371/journal.pbio.0020296. PMC 514491. PMID 15328540.
- ^ Kaeberlein, M; Powers Rw, III; Steffen, K. K.; Westman, E. A.; Hu, D; Dang, N; Kerr, E. O.; Kirkland, K. T.; Fields, S; Kennedy, B. K. (2005). "Regulation of yeast replicative life span by TOR and Sch9 in response to nutrients". Science. 310 (5751): 1193–6. Bibcode:2005Sci...310.1193K. doi:10.1126/science.1115535. PMID 16293764. S2CID 42188272.
- ^ "Past Winners".
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