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Margaret Lee (c. 1506 – c. 1543) was a sister of the poet Thomas Wyatt, and likely a friend of Queen Anne Boleyn, second wife of Henry VIII of England. She was the daughter of Sir Henry Wyatt and Anne Skinner, and was married to Sir Anthony Lee (died 1549), by whom she had four sons and five daughters. Historians have speculated that she was present during Boleyn's execution.Painting: Workshop of Hans Holbein.