Talk:Polynucleotide phosphorylase

Bifunctional?

I wouldn't call it a bifunctional enzyme. A bifunctional enzyme is e.g. HCV NS3 which has a protease domain as well as helicase domains.

The two activities of PNP described here are merely reverse reactions of each other - they are carried out by the same catalytic center of the protein.

--Felix Tritschler (talk) 23:13, 28 February 2013 (UTC)

inconsistent distribution of the enzyme ! !

Once it is present in all species up to human, these are the phrases that infer this:

- It is involved on mRNA processing and degradation in bacteria, plants,[3] and in humans.[4]

- In humans, the enzyme is encoded by the PNPT1 gene.

second time it is present only in some eukaryotic cells:

- The protein is present in bacteria and in the chloroplasts[2] and mitochondria[5] of some eukaryotic cells.

moreover this line raise a question whether he means only some of human cells have the enzyme (in fact: the question whether he means some species &/or some tissues ?)

third time frankly stated that in human there is another related complex (didn't say: "together with the enzyme" or "in which the enzyme exist") which infer that in human the enzyme do not exist and a correlated complex take place:

- In eukaryotes and archaea, a structurally and evolutionary related complex exists, called the exosome.[5]

Yasir muhammed ali (talk) 12:03, 12 April 2016 (UTC)

Role in humans

It is not entirely clear from the article what the role of this enzyme is in humans. It is somehow involved in RNA processing or so it is written, but this should be clarified whenever someone has more information. 2A02:8388:1600:A200:3AD5:47FF:FE18:CC7F (talk) 19:11, 16 June 2022 (UTC)