Talk:Inverse polymerase chain reaction
Latest comment: 11 years ago by 50.195.10.169 in topic Diagram
![]() | This article is rated Start-class on Wikipedia's content assessment scale. It is of interest to the following WikiProjects: | ||||||||||||||||
|
Diagram
editthe diagram is incorrect - it contains an unnecessary restriction digest post-loop formation. PCR can be carried out on circular DNA!!!
- You're right, it's not necessary and should probably be changed. In my experience, however, linearizing circular templates sometimes increases the reaction efficiency, especially with larger products. – ClockworkSoul 17:09, 12 February 2010 (UTC)
as a scientist who not only does pcr, but was possibly the 1st person at MIT to do pcr, this article is worse then useless, imo - and I know how to talk to non scientists. scrap it and start over The essential point is that one starts with a circular piece of DNA and converts that to a linear with the primers at the end; from that you can talk about uses etc — Preceding unsigned comment added by 50.195.10.169 (talk) 20:52, 18 July 2013 (UTC)