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Pokeweed mitogen is a mitogen derived from the roots of Phytolacca americana. It functions as a lectin and a mitotic stimulus for the division of lymphocytes.
Biological Functions
editThis lectin specifically induces the proliferation of B cells, plasma cells and T cells.[1] This lectin contains Toll-like receptor 4 (TLR4) proteins which signals for the B-cell for a proliferative response. B cells are shown to have no response to pure PWM without the Toll-like receptor suggesting that PWM inducing ability relies on TLR4 protein's presence for the signaling of B-cells and the response.[2]
Research
editPWM can contribute to B-cell assay or other lymphocytes for testing. The amplified number of proliferated cells makes it easier to test different lymphocyte functions, biological mechanisms, and response to different stimuli.[2] Pokeweed mitogen is also used in research studies to stimulate different lymphocytes and observe change. When looking at deficiency of CD26 in knockout mice, after stimulation with PWM the role of CD26 in development of CD4+ T cells was observed.[3] Another study where PWM was used as a stimulus was in the proteome analysis of lymphocytes of different immune phenotypes in cows.[4] Other research involves the PWM lectin playing a role in cancer detection. The PWM lectin was found to have affinity for human colorectal cancers indicating its usefulness in analysis of glycoconjugates found in colorectal cancer and detection of cancerous tissue.[5]
Clinical Significance
editPokeweed mitogen has the ability to play a role in immunology. It can test for immunodeficiency by inducing cell proliferation and test for abnormal counts of lymphocytes.[2] It can also induce proliferation of cells that target tumors, which is useful for the treatment of cancer.[6] Other studies in mice showed that PWM contributes to weight-loss effects and increased glucose uptake which makes this lectin a subject for implementation in diabetes or obesity in humans.[7]
References
edit- ^ Assenmacher, Mario; Avraham, Hava Karsenty; Avraham, Shalom; Bala, Shukal, eds. (2005), "Pokeweed Mitogen", Encyclopedic Reference of Immunotoxicology, Berlin, Heidelberg: Springer, p. 509, doi:10.1007/3-540-27806-0_1183, ISBN 978-3-540-27806-1, retrieved 2021-11-30
- ^ a b c Bekeredjian-Ding I, Foermer S, Kirschning CJ, Parcina M, Heeg K (2012-01-04). "Poke weed mitogen requires Toll-like receptor ligands for proliferative activity in human and murine B lymphocytes". PLOS ONE. 7 (1): e29806. Bibcode:2012PLoSO...729806B. doi:10.1371/journal.pone.0029806. PMC 3251602. PMID 22238657.
- ^ Yan, Shuling; Marguet, Didier; Dobers, Jörg; Reutter, Werner; Fan, Hua (2003). "Deficiency of CD26 results in a change of cytokine and immunoglobulin secretion after stimulation by pokeweed mitogen". European Journal of Immunology. 33 (6): 1519–1527. doi:10.1002/eji.200323469. ISSN 0014-2980. PMID 12778469. S2CID 13371173.
- ^ Kleinwort, Kristina J. H.; Degroote, Roxane L.; Hirmer, Sieglinde; Korbonits, Lucia; Lorenz, Lea; Scholz, Armin M.; Hauck, Stefanie M.; Deeg, Cornelia A. (2022-02-08). "Bovine Peripheral Blood Derived Lymphocyte Proteome and Secretome Show Divergent Reaction of Bovine Immune Phenotypes after Stimulation with Pokeweed Mitogen". Proteomes. 10 (1): 7. doi:10.3390/proteomes10010007. ISSN 2227-7382. PMC 8883952. PMID 35225986.
- ^ Aoki, T; Kawano, J; Oinuma, T; Haraguchi, Y; Eto, T; Suganuma, T (1993). "Human colorectal carcinoma-specific glycoconjugates detected by pokeweed mitogen lectin". Journal of Histochemistry & Cytochemistry. 41 (9): 1321–1330. doi:10.1177/41.9.8102627. ISSN 0022-1554. PMID 8102627. S2CID 26077790.
- ^ Numa K, Tani T, Kodama M (1990). "Trial of anticancer immunotherapy with immobilized pokeweed mitogen: immunotherapy by extracorporeal circulation". Cancer Immunology, Immunotherapy. 32 (2): 125–130. doi:10.1007/BF01754209. PMC 11038877. PMID 2289204. S2CID 26066215.
- ^ Lazarus, Dougals D.; Trimble, Linda A.; Moldawer, Lyle L. (1998-01-01). "The metabolic effects of pokeweek mitogen in mice". Metabolism: Clinical and Experimental. 47 (1): 75–82. doi:10.1016/S0026-0495(98)90196-2. ISSN 0026-0495. PMID 9440481.
External links
edit- Pokeweed+mitogens at the U.S. National Library of Medicine Medical Subject Headings (MeSH)