In biology, the PYLIS downstream sequence (PYLIS: pyrrolysine insertion sequence) is a stem-loop structure that appears on some mRNA sequences. This structural motif was previously thought to cause the UAG (amber) stop codon to be translated to the amino acid pyrrolysine instead of ending the protein translation.[1][2][3] However, it has been shown that PYLIS has no effect upon the efficiency of the UAG suppression,[4] hence even its name is, in fact, incorrect.
| Pyrrolysine insertion sequence 1 | |
|---|---|
 Predicted secondary structure of PYLIS 1 RNA. | |
| Identifiers | |
| Symbol | PYLIS 1 |
| Rfam | RF01982 |
| Other data | |
| RNA type | Cis-regulatory element |
| Domain(s) | Archaea |
| PDB structures | PDBe |
See also
editReferences
edit- ^ Namy O, Rousset JP, Napthine S, Brierley I (2004). "Reprogrammed genetic decoding in cellular gene expression". Molecular Cell. 13 (2): 157–168. doi:10.1016/S1097-2765(04)00031-0. PMID 14759362.
- ^ Théobald-Dietrich A, Giegé R, Rudinger-Thirion J (2005). "Evidence for the existence in mRNAs of a hairpin element responsible for ribosome dependent pyrrolysine insertion into proteins". Biochimie. 87 (9–10): 813–817. doi:10.1016/j.biochi.2005.03.006. PMID 16164991.
- ^ Zhang Y, Baranov PV, Atkins JF, Gladyshev VN (May 2005). "Pyrrolysine and selenocysteine use dissimilar decoding strategies". J. Biol. Chem. 280 (21): 20740–20751. doi:10.1074/jbc.M501458200. PMID 15788401.
- ^ Namy, Olivier; Zhou, Yu; Gundllapalli, Sarath; Polycarpo, Carla R.; Denise, Alain; Rousset, Jean-Pierre; Söll, Dieter; Ambrogelly, Alexandre (November 2007). "Adding pyrrolysine to the Escherichia coli genetic code". FEBS Letters. 581 (27): 5282–5288. doi:10.1016/j.febslet.2007.10.022. PMID 17967457.
Further reading
edit- Longstaff DG, Blight SK, Zhang L, Green-Church KB, Krzycki JA (January 2007). "In vivo contextual requirements for UAG translation as pyrrolysine". Mol. Microbiol. 63 (1): 229–241. doi:10.1111/j.1365-2958.2006.05500.x. PMID 17140411. S2CID 25346794.
- Theil Have, C; Zambach, S; Christiansen, H (April 4, 2013). "Effects of using coding potential, sequence conservation and mRNA structure conservation for predicting pyrrolysine containing genes". BMC Bioinformatics. 14 (1): 118. doi:10.1186/1471-2105-14-118. PMC 3639795. PMID 23557142.
External links
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