"Killer Queen" is a 1974 song by the British rock band Queen.
Killer Queen may also refer to:
- "Killer Queen" (Family Guy), an episode of the animated sitcom Family Guy which features the aforementioned song
- Killer Queen: A Tribute to Queen, a tribute album to the band Queen
- Killer Queen (video game), a 2013 video game
- Killer Queen (drag queen) (born 1989), Spanish drag queen
- Killer Queen, a Stand power used by the main antagonist Yoshikage Kira in Diamond Is Unbreakable from JoJo's Bizarre Adventure
- The Babysitter: Killer Queen, a 2020 American black comedy slasher film