Hydroxyisourate hydrolase

In enzymology, a hydroxyisourate hydrolase (EC 3.5.2.17) is an enzyme that catalyzes the chemical reaction

Hydroxyisourate hydrolase
Identifiers
EC no.	3.5.2.17
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

5-hydroxyisourate + H₂O $\rightleftharpoons$ 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Thus, the two substrates of this enzyme are 5-hydroxyisourate and H₂O, whereas its product is 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-hydroxyisourate amidohydrolase. Other names in common use include HIUHase, and 5-hydroxyisourate hydrolase. This enzyme participates in purine metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2H1X and 2H6U.

See also

• 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase

References

• Raychaudhuri A, Tipton PA (2003). "A familiar motif in a new context: the catalytic mechanism of hydroxyisourate hydrolase". Biochemistry. 42 (22): 6848–52. doi:10.1021/bi034137o. PMID 12779339.
• Raychaudhuri A, Tipton PA (2002). "Cloning and expression of the gene for soybean hydroxyisourate hydrolase. Localization and implications for function and mechanism". Plant Physiol. 130 (4): 2061–8. doi:10.1104/pp.011049. PMC 166717. PMID 12481089.
• Sarma AD, Serfozo P, Kahn K, Tipton PA (1999). "Identification and purification of hydroxyisourate hydrolase, a novel ureide-metabolizing enzyme". J. Biol. Chem. 274 (48): 33863–5. doi:10.1074/jbc.274.48.33863. PMID 10567345.