Draft:Methionylglutaminylarginyltyrosylglutamyl...serine

• Comment: Last chance: provide references to reliable sources or the draft gets deleted. Tagishsimon (talk) 21:12, 26 October 2023 (UTC)

• Comment: Fails WP:GNG, lacks any sources or references. Dan arndt (talk) 03:27, 25 May 2023 (UTC)

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Methionylglutaminylarginyltyrosylglutamylserylleucylphenylalanylalanylglutaminylleucyllysylglutanylarginyllysylglutamylglysylalanylphenylalanylvalylprolylphenylalanylyalylthreonylleucylglcycylaspartylprolylglicylisoleucyglutamylglutamimlserylleucyllysylisoleucylaspartylthreonylleucylisoleucylglutamylalanylglyclyalanylaspartylalanylleucyglutamylleucylgluycylisoleucylproluylphenylalanyserylaspartyprolylleucelalanylaspartylglycylprolylthreonylisolleucyglutaminylasparagimylalanythreonylleucylarginylalanylphenylalanylalanylalanylglycylvalylthreonylprolylalanylglutaminylcysteinylphenylalanylglglutamylmthionylleucalanylleucylisoleucylarginylglutaninyllysylhistidylpooyuthreonyoisoleucylprolylisoleuclglycylleucylleucylmethiolnyltyrosylalanylasbaraginylleucylvalylphenylalanylsparaginyyllysylglycylisoleucylaspartylglutamylphenylalanylyltyrosylalanylglutaminylcysteinylglutamyllysylvalylglycylvalylspartylserylvalylleucylvallalanylaspartylvalylprolylvalvlglutaminylglutamylserylalanylprolyiphenylalalrginylglutaminylalanylalanylleucylarginylhistidylasparaginylvalylalalprlylisoleucylphenylalanylisoleucylcysteinylprolyprolylaspartylalanylaspartyspartyleucylleucylarginylglutaminylisoleucylalanylseryltyroslglycylarginylglycyltyrosylthreonyltyrosylleucylleucylserylarginlalanylglycylvalylthreonylglycylalanylglutamylasparaginylarginylanylalanylleucylprolylleucylaspaaginylhitidylleucylvalylalanyllysylleucyllysylglutamyltyrosylasparagimylalanylalanyprolylprolylleucylglutaminylglycyphenlalanylglycylisoleyucylserylalanylprolylaspartymgmutaminylvalyllysylalanylalanylisoleucylalspartylalanylglycylalanylalanylglycylalanylasoleucylserylglycylserylalanylisoleucylbalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginlisoleucylglutamylpronylgluotamyllysylmethionylluecylalanylalanyoeucyllysylvalylphenylalanlvalylglutamilylprolylmethionyllysylalanylalanylthreonylarginylserine. is the chemical name for the tryptophan synthase alpha subunit of Escherichia coli (E. coli). It is an essential enzyme involved in the biosynthesis of the amino acid tryptophan, a building block of proteins. TrpA is part of a heteromeric complex known as tryptophan synthase, which also includes the beta subunit encoded by the trpB gene.

English

Pronunciation: Methionylglutaminylarginyltyrosylglutamylserylleucylphenylalanylalanylglutaminylleucyllysylglutanylarginyllysylglutamylglysylalanylphenylalanylvalylprolylphenylalanylyalylthreonylleucylglcycylaspartylprolylglicylisoleucyglutamylglutamimlserylleucyllysylisoleucylaspartylthreonylleucylisoleucylglutamylalanylglyclyalanylaspartylalanylleucyglutamylleucylgluycylisoleucylproluylphenylalanyserylaspartyprolylleucelalanylaspartylglycylprolylthreonylisolleucyglutaminylasparagimylalanythreonylleucylarginylalanylphenylalanylalanylalanylglycylvalylthreonylprolylalanylglutaminylcysteinylphenylalanylglglutamylmthionylleucalanylleucylisoleucylarginylglutaninyllysylhistidylpooyuthreonyoisoleucylprolylisoleuclglycylleucylleucylmethiolnyltyrosylalanylasbaraginylleucylvalylphenylalanylsparaginyyllysylglycylisoleucylaspartylglutamylphenylalanylyltyrosylalanylglutaminylcysteinylglutamyllysylvalylglycylvalylspartylserylvalylleucylvallalanylaspartylvalylprolylvalvlglutaminylglutamylserylalanylprolyiphenylalalrginylglutaminylalanylalanylleucylarginylhistidylasparaginylvalylalalprlylisoleucylphenylalanylisoleucylcysteinylprolyprolylaspartylalanylaspartyspartyleucylleucylarginylglutaminylisoleucylalanylseryltyroslglycylarginylglycyltyrosylthreonyltyrosylleucylleucylserylarginlalanylglycylvalylthreonylglycylalanylglutamylasparaginylarginylanylalanylleucylprolylleucylaspaaginylhitidylleucylvalylalanyllysylleucyllysylglutamyltyrosylasparagimylalanylalanyprolylprolylleucylglutaminylglycyphenlalanylglycylisoleyucylserylalanylprolylaspartymgmutaminylvalyllysylalanylalanylisoleucylalspartylalanylglycylalanylalanylglycylalanylasoleucylserylglycylserylalanylisoleucylbalyllysylisoleucylisoleucylglutamylglutaminylhistidylasparaginlisoleucylglutamylpronylgluotamyllysylmethionylluecylalanylalanyoeucyllysylvalylphenylalanlvalylglutamilylprolylmethionyllysylalanylalanylthreonylarginylserine.

Structure and Function

E. coli TrpA is a multifunctional protein with a molecular weight of approximately 27 kilodaltons. It is composed of 268 amino acids and is encoded by the trpA gene located on the bacterial chromosome. The protein consists of several distinct domains that contribute to its various functions.

The primary role of TrpA is to catalyze the conversion of indole-3-glycerol phosphate and L-serine to indole, glyceraldehyde 3-phosphate, and water. This reaction represents the first step in the tryptophan biosynthetic pathway. TrpA possesses both pyridoxal phosphate (PLP) binding and catalytic domains, which are essential for its enzymatic activity.

Regulation

The expression of the trpA gene and subsequent synthesis of TrpA is tightly regulated in E. coli. The availability of tryptophan in the cellular environment plays a critical role in this regulation. When tryptophan levels are high, it acts as a corepressor by binding to the trp repressor protein, resulting in the repression of trpA gene expression.

Under conditions of tryptophan limitation, the trp repressor is unable to bind to the trp operator region, allowing RNA polymerase to transcribe the trpA gene. This mechanism ensures that the E. coli cell produces tryptophan synthase only when tryptophan is scarce.

Importance in Biological Systems

Tryptophan synthase is a vital enzyme in both prokaryotic and eukaryotic organisms as it enables the de novo biosynthesis of tryptophan, an essential amino acid. Tryptophan serves as a precursor for various important molecules, including neurotransmitters, metabolites, and cofactors.

In E. coli, TrpA plays a crucial role in maintaining cellular homeostasis by regulating tryptophan levels. It contributes to the overall metabolic balance and ensures an adequate supply of tryptophan for protein synthesis and other cellular processes.