ARFGEF2

Brefeldin A-inhibited guanine nucleotide-exchange protein 2 is a protein that in humans is encoded by the ARFGEF2 gene.

ARFGEF2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3L8N, 3SWV
Identifiers
Aliases	ARFGEF2, BIG2, PVNH2, dJ1164I10.1, ADP ribosylation factor guanine nucleotide exchange factor 2
External IDs	OMIM: 605371; MGI: 2139354; HomoloGene: 111880; GeneCards: ARFGEF2; OMA:ARFGEF2 - orthologs
Gene location (Human) Chr. Chromosome 20 (human) Band 20q13.13 Start 48,921,711 bp End 49,036,693 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2|2 H3 Start 166,647,508 bp End 166,739,972 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in cartilage tissue jejunal mucosa parotid gland Epithelium of choroid plexus corpus epididymis skin of thigh cardia mucosa of sigmoid colon secondary oocyte retinal pigment epithelium Top expressed in retinal pigment epithelium lacrimal gland secondary oocyte submandibular gland Epithelium of choroid plexus stroma of bone marrow superior cervical ganglion epithelium of stomach interventricular septum medial vestibular nucleus More reference expression data BioGPS More reference expression data
Gene ontology Molecular function guanyl-nucleotide exchange factor activity GABA receptor binding myosin binding protein binding protein kinase A regulatory subunit binding Cellular component cytoplasm recycling endosome cytosol endosome Golgi apparatus cell projection membrane Golgi membrane dendritic spine synapse microtubule organizing center cell junction trans-Golgi network dendrite symmetric synapse asymmetric synapse perinuclear region of cytoplasm cytoskeleton cytoplasmic vesicle axonemal microtubule Biological process endomembrane system organization intracellular signal transduction regulation of ARF protein signal transduction endosome organization receptor recycling positive regulation of tumor necrosis factor production protein transport Golgi to plasma membrane transport exocytosis transport regulation of molecular function vesicle-mediated transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10564 99371 Ensembl ENSG00000124198 ENSMUSG00000074582 UniProt Q9Y6D5 A2A5R2 RefSeq (mRNA) NM_006420 NM_001085495 RefSeq (protein) NP_006411 NP_001078964 Location (UCSC) Chr 20: 48.92 – 49.04 Mb Chr 2: 166.65 – 166.74 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.

Interactions

ARFGEF2 has been shown to interact with ARFGEF1, PRKAR1A and PRKAR2A.

External links

• Human ARFGEF2 genome location and ARFGEF2 gene details page in the UCSC Genome Browser.

Further reading

• Yamaji R, Adamik R, Takeda K, Togawa A, Pacheco-Rodriguez G, Ferrans VJ, Moss J, Vaughan M (Mar 2000). "Identification and localization of two brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors in a macromolecular complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (6): 2567–72. Bibcode:2000PNAS...97.2567Y. doi:10.1073/pnas.97.6.2567. PMC 15969. PMID 10716990.
• Kamei Y, Takeda Y, Teramoto K, Tsutsumi O, Taketani Y, Watanabe K (Oct 2000). "Human NB-2 of the contactin subgroup molecules: chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members". Genomics. 69 (1): 113–9. doi:10.1006/geno.2000.6310. PMID 11013081.
• Pacheco-Rodriguez G, Moss J, Vaughan M (2002). "BIG1 and BIG2: brefeldin A-inhibited guanine nucleotide-exchange proteins for ADP-ribosylation factors". G Protein Pathways - Part C, Effector Mechanisms. Methods in Enzymology. Vol. 345. pp. 397–404. doi:10.1016/S0076-6879(02)45032-X. ISBN 9780121822460. PMID 11665623.
• Shinotsuka C, Yoshida Y, Kawamoto K, Takatsu H, Nakayama K (Mar 2002). "Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation". The Journal of Biological Chemistry. 277 (11): 9468–73. doi:10.1074/jbc.M112427200. PMID 11777925.
• Shinotsuka C, Waguri S, Wakasugi M, Uchiyama Y, Nakayama K (Jun 2002). "Dominant-negative mutant of BIG2, an ARF-guanine nucleotide exchange factor, specifically affects membrane trafficking from the trans-Golgi network through inhibiting membrane association of AP-1 and GGA coat proteins". Biochemical and Biophysical Research Communications. 294 (2): 254–60. doi:10.1016/S0006-291X(02)00456-4. PMID 12051703.
• Li H, Adamik R, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2003). "Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2)". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1627–32. Bibcode:2003PNAS..100.1627L. doi:10.1073/pnas.0337678100. PMC 149883. PMID 12571360.
• Sheen VL, Ganesh VS, Topcu M, Sebire G, Bodell A, Hill RS, Grant PE, Shugart YY, Imitola J, Khoury SJ, Guerrini R, Walsh CA (Jan 2004). "Mutations in ARFGEF2 implicate vesicle trafficking in neural progenitor proliferation and migration in the human cerebral cortex". Nature Genetics. 36 (1): 69–76. doi:10.1038/ng1276. PMID 14647276.
• Colland F, Jacq X, Trouplin V, Mougin C, Groizeleau C, Hamburger A, Meil A, Wojcik J, Legrain P, Gauthier JM (Jul 2004). "Functional proteomics mapping of a human signaling pathway". Genome Research. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP (Aug 2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proceedings of the National Academy of Sciences of the United States of America. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Xu KF, Shen X, Li H, Pacheco-Rodriguez G, Moss J, Vaughan M (Feb 2005). "Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70". Proceedings of the National Academy of Sciences of the United States of America. 102 (8): 2784–9. Bibcode:2005PNAS..102.2784X. doi:10.1073/pnas.0409871102. PMC 549493. PMID 15705715.
• Lu J, Tiao G, Folkerth R, Hecht J, Walsh C, Sheen V (Jan 2006). "Overlapping expression of ARFGEF2 and Filamin A in the neuroependymal lining of the lateral ventricles: insights into the cause of periventricular heterotopia". The Journal of Comparative Neurology. 494 (3): 476–84. doi:10.1002/cne.20806. PMID 16320251. S2CID 16527134.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Islam A, Shen X, Hiroi T, Moss J, Vaughan M, Levine SJ (Mar 2007). "The brefeldin A-inhibited guanine nucleotide-exchange protein, BIG2, regulates the constitutive release of TNFR1 exosome-like vesicles". The Journal of Biological Chemistry. 282 (13): 9591–9. doi:10.1074/jbc.M607122200. PMID 17276987.
• Kuroda F, Moss J, Vaughan M (Feb 2007). "Regulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) and BIG2 activity via PKA and protein phosphatase 1gamma". Proceedings of the National Academy of Sciences of the United States of America. 104 (9): 3201–6. Bibcode:2007PNAS..104.3201K. doi:10.1073/pnas.0611696104. PMC 1805514. PMID 17360629.