2-deoxy-scyllo-Inosose synthase (EC 4.2.3.124, btrC (gene), neoC (gene), kanC (gene)) is an enzyme with systematic name D-glucose-6-phosphate phosphate-lyase (2-deoxy-scyllo-inosose-forming).[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction
| 2-deoxy-scyllo-inosose synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.3.124 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- D-glucose 6-phosphate 2-deoxy-L-scyllo-inosose + phosphate
This enzyme requires Co2+.
References
edit- ^ Kudo F, Yamauchi N, Suzuki R, Kakinuma K (May 1997). "Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans". The Journal of Antibiotics. 50 (5): 424–8. doi:10.7164/antibiotics.50.424. PMID 9207913.
- ^ Kudo F, Hosomi Y, Tamegai H, Kakinuma K (February 1999). "Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics". The Journal of Antibiotics. 52 (2): 81–8. doi:10.7164/antibiotics.52.81. PMID 10344560.
- ^ Kudo F, Tamegai H, Fujiwara T, Tagami U, Hirayama K, Kakinuma K (June 1999). "Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase". The Journal of Antibiotics. 52 (6): 559–71. doi:10.7164/antibiotics.52.559. PMID 10470681.
- ^ Huang Z, Kakinuma K, Eguchi T (April 2005). "Stereospecificity of hydride transfer in NAD+-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics". Bioorganic Chemistry. 33 (2): 82–9. doi:10.1016/j.bioorg.2004.09.002. PMID 15788164.
- ^ Thuy ML, Kharel MK, Lamichhane R, Lee HC, Suh JW, Liou K, Sohng JK (April 2005). "Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans". Biotechnology Letters. 27 (7): 465–70. doi:10.1007/s10529-005-2222-y. PMID 15928851.
- ^ Tamegai H, Nango E, Koike-Takeshita A, Kudo F, Kakinuma K (July 2002). "Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans". Bioscience, Biotechnology, and Biochemistry. 66 (7): 1538–45. doi:10.1271/bbb.66.1538. PMID 12224638.
- ^ Tamegai H, Sawada H, Nango E, Aoki R, Hirakawa H, Iino T, Eguchi T (2010). "Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism". Bioscience, Biotechnology, and Biochemistry. 74 (6): 1215–9. doi:10.1271/bbb.90961. PMID 20530911.
External links
edit- 2-deoxy-scyllo-inosose+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
