In molecular biology, the SR1 RNA is a small RNA (sRNA) produced by species of Bacillus and closely related bacteria.[1] It is a dual-function RNA which acts both as a protein-coding RNA and as a regulatory sRNA.
SR1 RNA is involved in the regulation of arginine catabolism. SR1 RNA binds to complementary stretches of ahrC mRNA (also known as argR and inhibits translation.[2][3] AhrC endodes an arginine repressor protein which represses synthesis of arginine biosynthetic enzymes and activates arginine catabolic enzymes via regulation of the rocABC and rocDEF operons.[4][5][6]
In addition to acting as a sRNA, SR1 also encodes a small peptide, SR1P. SR1P binds to glyceraldehyde-3-phosphate dehydrogenase (GapA) and stabilises the gapA operon mRNAs.[7]
See also
editReferences
edit- ^ Gimpel M, Preis H, Barth E, Gramzow L, Brantl S (October 13, 2012). "SR1—a small RNA with two remarkably conserved functions". Nucleic Acids Research. 40 (22): 11659–11672. doi:10.1093/nar/gks895. PMC 3526287. PMID 23034808.
- ^ Heidrich N, Moll I, Brantl S (2007). "In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA". Nucleic Acids Research. 35 (13): 4331–4346. doi:10.1093/nar/gkm439. PMC 1935000. PMID 17576690.
- ^ Heidrich N, Chinali A, Gerth U, Brantl S (October 2006). "The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism". Molecular Microbiology. 62 (2): 520–536. doi:10.1111/j.1365-2958.2006.05384.x. PMID 17020585.
- ^ North AK, Smith MC, Baumberg S (August 1, 1989). "Nucleotide sequence of a Bacillus subtilis arginine regulatory gene and homology of its product to the Escherichia coli arginine repressor". Gene. 80 (1): 29–38. doi:10.1016/0378-1119(89)90247-3. PMID 2507400.
- ^ Czaplewski LG, North AK, Smith MC, Baumberg S, Stockley PG (January 1992). "Purification and initial characterization of AhrC: the regulator of arginine metabolism genes in Bacillus subtilis". Mol. Microbiol. 6 (2): 267–275. doi:10.1111/j.1365-2958.1992.tb02008.x. PMID 1312212. S2CID 3219261.
- ^ Dennis CA, Glykos NM, Parsons MR, Phillips SE (March 2002). "The structure of AhrC, the arginine repressor/activator protein from Bacillus subtilis". Acta Crystallographica Section D. 58 (Pt 3): 421–430. doi:10.1107/s0907444901021692. PMID 11856827.
- ^ Gimpel M, Heidrich N, Mäder U, Krügel H, Brantl S (May 2010). "A dual-function sRNA from B. subtilis: SR1 acts as a peptide encoding mRNA on the gapA operon" (PDF). Molecular Microbiology. 76 (4): 990–1009. doi:10.1111/j.1365-2958.2010.07158.x. PMID 20444087.
- ^ Licht A, Preis S, Brantl S (October 2005). "Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis". Molecular Microbiology. 58 (1): 189–206. doi:10.1111/j.1365-2958.2005.04810.x. PMID 16164558. S2CID 29961832.
- ^ Licht A, Brantl S (October 2009). "The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters". Journal of Biological Chemistry. 284 (44): 30032–30038. doi:10.1074/jbc.M109.033076. PMC 2781557. PMID 19726675.
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