Talk:Movat's stain

Linedwell ^{[talk/@frwiki]} 19:50, 2 July 2016 (UTC)

Hi. I don't know how to edit Wikipedia. Can't remember my password anyway. I do know a lot about Pathology though and am an expert at the Movat Pentachrome stain. Although these days people use the Modified Movat's Pentachrome stain.

I have written out the basic principle of the stain to try to lift the article from stub-class. Could someone pleas take this information and add it to the article?

I also have much better images than the ones in the article. I stained the tissue took the images myself. I would like to offer them for the article, but don't really know how. I'm just a scientist after all.

I take issue with this article being considered of "low importance." The Movat stain is sometimes called "The Mother of all special stains." To my knowledge the stain cannot yet be automated, and must be performed by hand by a skilled histologist. There are many steps where a single error can ruin the stain. And it takes five hours to perform.

More importantly, cardiovascular disease is the primary cause of morbidity and mortality in the developed nations. The Movat's Pentachrome stain is an indispensable procedure for histopathological studies into cardiovascular disease, not least because of the wealth of information it provides about the composition of atherosclerotic lesions.

Anyway, here is my text.

Principle: Alcian Blue solution stains acid mucopolysaccharides blue to blue-green. Alcian blue is then converted to insoluble monastral fast blue using alkaline alcohol. Resorcin-fuchsin stains elastin black. Weigart’s Iron Hematoxylin stains nuclei black, A solution of Woodstain Scarlet and Acid Fuchsin preferentially stains muscle and reticular fibres. Differentiation with Phosphotungstic acid removes Woodstain Scarlet and Acid Fuchsin from ground substance, revealing the monastral fast blue again. Phosphotungstic acid is removed with acetic acid, acetic acid is removed by washing in water. Alcoholic Saffron stains collagen and reticular fibers yellow.

Source: Theory and Practice of Histotechnology, Second Edition. Published in 1980. Authors Dezna C. Sheehan and Barbara B. Hrapchak

Publisher:Batelle Press.

ISBN 1-57477-067-5

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