Procyclins also known as procyclic acidic repetitive proteins or PARP[1] are proteins developed in the surface coating of Trypanosoma brucei parasites while in their tsetse fly vector.[2] The cell surface of the bloodstream form features a dense coat of variable surface glycoproteins (VSGs) which is replaced by an equally dense coat of procyclins when the parasite differentiates into the procylic form in the tsetse fly midgut.
| Procyclic acidic repetitive | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | ? | ||||||||
| Pfam | PF05887 | ||||||||
| InterPro | IPR008882 | ||||||||
| |||||||||
| EP1 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | Tb10.6k15.0020 | ||||||
| Entrez | 3661797 | ||||||
| RefSeq (mRNA) | XM_818154.1 | ||||||
| RefSeq (Prot) | XP_823247.1 | ||||||
| UniProt | Q389V1 | ||||||
| Other data | |||||||
| Chromosome | 10: 2.48 - 2.48 Mb | ||||||
| |||||||
| Surface protein EP1-2 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | EP1-2 | ||||||
| UniProt | Q7KG35 | ||||||
| |||||||
| EP2 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | Tb10.6k15.0030 | ||||||
| Entrez | 3661534 | ||||||
| RefSeq (mRNA) | XM_818153.1 | ||||||
| RefSeq (Prot) | XP_823246.1 | ||||||
| UniProt | Q389V2 | ||||||
| Other data | |||||||
| Chromosome | 10: 2.48 - 2.48 Mb | ||||||
| |||||||
| Surface protein EP2-1 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | EP2-1 | ||||||
| UniProt | Q95PJ2 | ||||||
| |||||||
| Procyclin EP3 | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | EP3 | ||||||
| UniProt | Q86MA3 | ||||||
| |||||||
| EP3-2 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | Tb927.6.520 | ||||||
| Entrez | 3657688 | ||||||
| RefSeq (mRNA) | XM_840082.1 | ||||||
| RefSeq (Prot) | XP_845175.1 | ||||||
| UniProt | Q581F6 | ||||||
| Other data | |||||||
| Chromosome | 6: 0.23 - 0.23 Mb | ||||||
| |||||||
| Surface protein EP3-3 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | EP3-3 | ||||||
| UniProt | Q95NW2 | ||||||
| |||||||
| Surface protein EP3-4 procyclin | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | EP3-4 | ||||||
| UniProt | Q95PJ3 | ||||||
| |||||||
| GPEET2 procyclin precursor | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | Tb927.6.510, Tb06.28F21.90 | ||||||
| Entrez | 3657686 | ||||||
| RefSeq (mRNA) | XM_840081.1 | ||||||
| RefSeq (Prot) | XP_845174.1 | ||||||
| UniProt | Q581F9 | ||||||
| Other data | |||||||
| Chromosome | 6: 0.23 - 0.23 Mb | ||||||
| |||||||
There are six or seven procyclin genes that encode unusual proteins with extensive tandem repeat units of glutamic acid (E) and proline (P), referred to as EP repeats (EP1, EP1-2, EP2, EP2-1, EP3, EP3-2, EP3-4), and two genes that encode proteins with internal pentapeptide GPEET repeats (GPEET2).[3]
EP1 is a 141 amino acids protein and EP2 is a 129 AA protein. Both proteins have their coding genes situated on chromosome 10. GPEET2 is a 114 AA protein and EP3-2 is 123 AA protein with genes situated on chromosome 6.
See also
editReferences
edit- ^ Rudenko G, Le Blancq S, Smith J, Lee MG, Rattray A, Van der Ploeg LH (July 1990). "Procyclic acidic repetitive protein (PARP) genes located in an unusually small alpha-amanitin-resistant transcription unit: PARP promoter activity assayed by transient DNA transfection of Trypanosoma brucei". Mol. Cell. Biol. 10 (7): 3492–504. doi:10.1128/MCB.10.7.3492. PMC 360784. PMID 1694012.
- ^ Acosta-Serrano A, Vassella E, Liniger M, Kunz Renggli C, Brun R, Roditi I, Englund PT (February 2001). "The surface coat of procyclic Trypanosoma brucei: programmed expression and proteolytic cleavage of procyclin in the tsetse fly". Proc. Natl. Acad. Sci. U.S.A. 98 (4): 1513–8. Bibcode:2001PNAS...98.1513A. doi:10.1073/pnas.98.4.1513. PMC 29288. PMID 11171982.
- ^ Bütikofer P, Ruepp S, Boschung M, Roditi I (September 1997). "'GPEET' procyclin is the major surface protein of procyclic culture forms of Trypanosoma brucei brucei strain 427". Biochem. J. 326 (2): 415–23. doi:10.1042/bj3260415. PMC 1218686. PMID 9291113.
External links
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