English: (A) Scanning electron micrograph of a micro-dissected sheep lens from a lamb less than a year old. Cells pull apart cleanly with membranes predominantly left intact. The capsule is very thin here, probably less than 2µm, as shown by the sheared edge being rounded but still appearing thin. The striped pattern near where the ligaments attach to the lens were not seen in large areas of well preserved thicker capsule elsewhere in the lens. Magnification bar 100µm (B) Transmission electron micrograph of lens capsule removed from a mouse, fixed and stained with heavy metals prior to ultra-thin sectioning. Top right is a copper supporting grid bar with adjacent darkly stained elongated cell nuclei. Circular white vesicles surround the nuclei and extend to the left and down into a band of the grayer cytoplasm. Further to the left is a nominally 10µm thick more lightly stained basement membrane with cytoplasmic feet and fibrils extending into and through it. (C) Interference contrast microscopy above and FITC coupled WGA fluorescence microscopy below can show structural features that parallel structures seen with electron microscopy. (D) A 3D highly stylized foot-feet model of basement membrane growth in a nominally 0.8mm diameter spherical 8 week old mouse lens based on Fig. 1C. The cells may form the foot from either the epithelium or the cells migrating along the ligaments towards the lens surface where they move to areas where they are needed. Blue spheres represent nuclei inside a cell. Smaller white spheres represent vesicles. The rows of numerous feet are highlighted by making them darker than the surrounding gray foot.
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Captions
Micrographs and 3D drawing of a "foot" structures on eye lens capsules