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Acremonium strictum

(Redirected from Cephalosporium acremonium)

Acremonium strictum is an environmentally widespread saprotroph species found in soil, plant debris, and rotting mushrooms.[1] Isolates have been collected in North and Central America, Asia, Europe and Egypt.[2] A. strictum is an agent of hyalohyphomycosis and has been identified as an increasingly frequent human pathogen in immunosuppressed individuals, causing localized, disseminated and invasive infections.[2][3][4] Although extremely rare, A. strictum can infect immunocompetent individuals, as well as neonates.[3][4] Due to the growing number of infections caused by A. strictum in the past few years, the need for new medical techniques in the identification of the fungus as well as for the treatment of human infections has risen considerably.[5]

Acremonium strictum
Scientific classification Edit this classification
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Sordariomycetes
Order: Hypocreales
Family: Hypocreaceae
Genus: Acremonium
Species:
A. strictum
Binomial name
Acremonium strictum
W.Gams (1971)
Synonyms
  • Cephalosporium acremonium Corda (1839)
  • Haplotrichum acremonium (Corda) Pound & Clem. (1896)
  • Hyalopus acremonium (Corda) M.A.J.Barbosa (1941)
  • Sarocladium strictum (Gams) Summerbell (2011)

Acremonium strictum has been shown to be involved in some myoparasitic relationships, as well as a wide range of plant endophytic and parasitic relationships,[6][7][8][9] and further studies are required to determine A. strictum's use as a biological control agent and role as a parasite that reduces crop yields. A. strictum exhibits metabolism of many products that imply future agricultural and pharmaceutical significance.[10][11]

General description

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The genus Acremonium is a large polyphyletic genus of approximately 150 species, many of which are derived from a closely related families in the Sordariomycetes.[12] The genus includes many slow growing, simply structured, anamorphic filamentous fungi,[3][5][12] typically encountered in wet, cellulose-based building materials suffering form chronic wet conditions.[13] Characteristic morphology in this genus is septate hyphae giving rise to thin, tapered aculeate phialides that are usually unicellular, or weakly branched conidiophores.[1][5][12] Human infections, though rare, usually occur in severely immunodeficient individuals.[2] A. strictum is mostly known to be involved with myparasitic relationships, as well as being a plant parasite and endophyte.[6][14][15]

Morphological identification

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Colonial appearance

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Acremonium strictum grows readily at 30 °C on glucose peptone agar, showing mycelium of approximately 50mm in size in 7 days. Colonies are flat, with smooth, wet, velvety or floccose texture, sometimes resembling thin cottony mounds.[16] The colour of mycelia ranges widely from light pink to orange, and sometimes yellow, white or green.[5][16] A. strictum filaments are sometimes bound together into ropes several cells in diameter.[1] Conidia grow as wet clusters or dry chains,[1] and grains produced are white to pale-yellow, soft and variable in shape.[1] Subcultures of the fungus can also be grow within seven days into smooth, moist, pink mycelia that resemble thin cotton.[5][17]

Microscopic appearance

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Conidia and conidiophores of the fungus Acremonium falciforme PHIL 4168 lores

Under the microscope at 30 °C, A. strictum shows long slender phialides, and conidia are cylindrical or ellipsoidal, formed in slimy bundles at the tips of the phialides. Lower microscopy shows pin-head spore ball formation.[16]

Species of Acremonium are morphologically very similar, making identification difficult. Shown in the image is a microscopic image of A. falciforme, an example showing the morphological similarities to A. strictum. Cases involving different species of Acremonium are often reported as simply as an Acremonium species, which reduces the amount of accurate information on the clinical presentation of A. strictum.[5] Isolates of phylogenetically remote species of Acremonium show considerable convergence.[12] As a human pathogen, diagnosis is made in isolation and identification of the fungus from granules in tissue and the presence of hyphae in microscopic examination of cutaneous biopsy and discharge.[1][3]

Genera that are morphologically similar to Acremonium include Fusarium, Phaeoacremonium, Verticillium, Phialemonium, and Lecanicillium.[5]

Genetic identification

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Identification of A. strictum isolates has shown that this fungus is phenotypically diverse and may vary genetically.[17] Due to phylogenetic ambiguities, an unknown proportion of the literature on A. strictum is based on studies of Acremonium sclerotigenum.[12] The fungus can generally be successfully identified by the nuclear ITS region sequence analysis.[5][18] Analysis of the genes for ribosomal large subunit (LSU) and whole small subunit (SSU) also help to elucidate phylogenetic relationships, since these genes are more conserved and less subject to evolutionary changes.[12] The species A. strictum is separated into three genogroups. Genogroup I is represented by type strain CBS 346.70, genogroup II by UW836 and genogroup III by UWFP940. These genogroups were determined based on GenBank entries for A. strictum.

Pathophysiology

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Human infections of Acremonium strictum are very rare, and usually develop after traumatic inoculation of the fungus.[5] Hyalophomycosis may occur in immunodeficient individuals, presented in the infected tissue by hyaline or colourless hyphae.[1][4] Peritonitis and pleuritis have resulted from A. strictum infections,[19][20] but cutaneous and subcutaneous infections of A. strictum are rarely reported.

  • Most human infections have been reported to occur in immunocompromised patients and have been presented as localized or disseminated, fungemia, mycetoma or ocular infections,[2][4] and often result in fatal cases.[3] A. strictum may result in invasive infections such as pneumonia, arthritis, osteomyelitis, endocarditis, meningitis and sepsis in immunodeficient patients.[3][19]
  • Infections in immunocompetent individuals usually follows inoculation during penetration of the extremities and cornea, resulting in localized infections.[3] The fungus can also cause onychomycosis, otomycosis and burn wound infection in immunocompetent individuals.[3] Patients with prosthetic valves who are infected with A. strictum in the region of the valve may suffer from severe inflammation, resulting in sepsis and multi-organ failure.[18]
  • Infections in neonates, although rare, can occur and be fatal.[4]

Many environmental factors such as the density of fungi in soil, rainfall, temperature, humidity and types of vegetation in close contact are relevant in determining the likelihood of acquiring hyalohypjomycosis infection by A. strictum.[3] Frequent exposure to contaminated water along with high temperature and humid environments increases the risk of infection.[3]

Clinical presentation and treatments

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Clinical presentation of an infection is ill-defined, but most individuals may present a skin rash and flu like symptoms, such as elevated body temperature and fatigue.[1][3] In more severe infections, such as in immunodeficient individuals, peritonitis and pleuritis, and may lead to multi-organ failure.[19][20] In the case of invasive infections, surgical intervention may be required to remove fungal mass from body tissues.[4] Due to limited, ill-defined cases and the variance in clinical presentation and species identification, no optimal treatments are available.[2] A. strictum and other Acremonium species are generally resistant to most antifungals, but antifungal susceptibility testing is recommended to select the most appropriate treatment for the strain of A. strictum that is the infection agent.[3] Amphotericin B therapy coupled with ketoconazole is usually recommended as the best available treatment.[2][3]

Fungal interactions

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Helminthosporium solani

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Acremonium strictum is generally known as a mycoparasite, as shown in its antagonistic relationship with Helminthosporium solani. H. solani is a potato (Solanum tuberosum) associated fungus, that has caused extreme and widespread losses in all market classes of potatoes since emerging in the United States. Commonly referred to as silver scurf, H. solani causes blemishes that decreases the quality of the crop, making it unfit for marketing. In more severe cases, H. solani causes weight loss in potatoes and creates lesions in the periderm, creating entry points for other tuber pathogens. In pure cultures of H. solani, isolates show white sectoring and rings, differential coloration and reduced sporulation in culture. Upon infection of A. strictum, cultures of H. solani were uniformly black, without white sectors or rings. A. strictum was able to significantly reduce sporulation of H. solani by 30%, spore germination by 20%, and mycelial growth 8% in culture. This evidence suggests that A. strictum may be used as a biological control agent against H. solani, which would greatly increase potato crop yields.[6]

Plant interactions

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Stalk rot

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Acremonium strictum is pathogenic to many monocotyledonous and dicotyledonous crops, causing leaf desiccation on one side of the midrib of these plants, plant wilt and abnormal, discoloured vasculature of the stalk near the soil line. Vasculature of the plant forms orange, red and brown bundles, usually resulting in death. Infection of A. strictum is systemic, and the fungus can be isolated from all tissues of the plant. Isolates have been found in plant seeds, which is probably the route of dissemination of the fungus. Crops affected by A. strictum include Acacia, Alnus, Ficus, Glycine, Gossypium, Triticum and Zea. Because of its ubiquitous presence in soils, A. strictum negatively impacts many agricultural plants, although more research is needed to investigate the parasitic interactions and develop strategies for its biological control.[14]

Root knot nematode

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Meloidogyne incognita is a polyphagous nematode that severely damages tomato crops by causing lesions in the roots by using a stylet, which allows other soil-dwelling fungal parasites to infect the host plant and cause complex disease interactions. A. strictum is reported to be a nematode egg parasite, as the eggs of M. incognita infested plants were found to be empty under A. strictum treatment. This treatment of A. strictum coupled with Trichoderma harzianum was found to be a very promising combination in the control of M. incognita in tomato plants.[15]

Fragaria ananassa

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It demonstrates a complicated relationship with strawberry host Fragaria ananassa,[7] in which the fungus may cause lesions and small necrotic, light-brown spots in leaves and petioles which increase as the disease progresses, adversely affecting strawberry crop.[21] Eventually the necrotic regions expand and cause the plant to wilt, but crown rot is not observed at any stage of the infection.[21] Although it appears to have a parasitic relationship with Fragaria ananassa, it also produces an elicitor protein, AsES, which provides systemic protection against anthracnose disease in strawberry host Fragaria ananassa, which shows a symbiotic relationship between the strawberry plant and Acremonium strictum.[7]

Atractylodes lancea

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Atractylodes lancea is a medicinal herb that grows in central China. A. strictum acts as a fungal endophyte and interacts with A. lancea in drought conditions and confers tolerance in moderate drought. Under mild drought conditions, A. strictum enhanced leaf soluble sugars, proteins, proline and antioxidant enzyme activity, which decreased the degree of plasmalemma oxidation. This increased A. lancea abscisic acid level and root:shoot ratio. While A. strictum may alleviate the effects of a mild to moderate drought, benefits of this endophytic relationship are constrained by drought degree, as there were no significant effects of A. strictum on A. lancea during periods of regular watering or severe drought.[8]

Maclura cochinchinensis

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In Maclura cochinchinensis, Acremonium strictum acts as an endophytic fungi that infects primarily the leaves of the plant. In this relationship, A. strictum was found to provide and mediate a protective response against herbivorous insects.[9]

Natural metabolites

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Acremostrictin

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Acremostrictin can be isolated from certain strains of A. strictum and is characterized as a highly oxygenated, tricyclic lactone metabolite.[22] This compound exhibits weak antibacterial properties against the bacterium Micrococcus luteus, Salmonella typhimurium and Proteus vulgaris. However, it had no effect on Bacillus subtilis, Staphylococcus aureus and Escherichia coli.[22] Acremostrictin has been shown to have concentration-dependent antioxidant activity, which conferred protection against oxidative stress induced cell death. Acremostrictin was shown to inhibit H2O2-induced death of human keratinocyte HaCaT cells. When extracted and isolated by filtration, acremostrictin presents as a colorless crystal solid.[22]

AsES

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AsES (A. strictum elicitor subtilisin; R4IR27) protein is an extracellular elicitor protein produced by A. strictum that provides complete systemic protection against anthracnose, cause by the fungal species Colletotrichum, in the natural host Fragaria ananassa as well as the non-natural host Arabidopsis thaliana. Anthracnose can affect all plant tissues, and appears as irregular and black leaf spot, flower blight, and fruit and crown rot, which results in serious losses in plant and fruit production. AsES has proteolytic activity that appears to elicit an immune response in these species that results in the accumulation of reactive oxygen species and the expression of defence related genes like PR1 and Chi2-1. Because it has been shown to provide the same systemic protection in non-natural hosts, this natural metabolite of A. strictum may be considered as a possible strategy for controlling anthracnose disease in plants.[7]

Cephalosporins

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Acremonium strictum produces some types of cephalosporins a group of antibiotics.

Industrial uses

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BMOs

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Biogenic Mn oxides (BMOs) are naturally occurring Mn oxides that have the ability to oxidize various redox-sensitive elements. A. strictum is a Mn(II)-oxidizing fungus that forms BMOs through the action of Mn(II) oxidase. In the presence of BMOs in buffer solutions with no additional nutrients, A. strictum is capable of sequestering high Mn(II) concentrations for at least 8 days, in which the amount of dissolved Mn(II) decreases rapidly in several hours and is converted to oxidized Mn(II). Deaeration of the buffer solution with N2 gas purging suppressed Mn(II) conversion, but this suppression is easily rescued by aeration, implying that dissolved oxygen is required for the Mn(II) sequestration and oxidation process. Adding NaN3, a toxic substance, also significantly reduces the sequestration rates of the fungal BMOs. Heat treatments revealed that temperatures below 85 °C do not alter the conformation of the Mn(II) oxidase in the BMOs. Freezing the fungal BMOs at −80 °C for 4 weeks did not affect the Mn(II) ability, and the reducible Mn was still dominated in solution. This makes fungal BMOs an effective Mn(II) sequestering material if needed. For example, it can be used for the continuous removal of Mn(II) from Mn(II) contaminated water without the need for any additives other than dissolved oxygen. The product is an oxide phase Mn(II) that would provide additional affinity for other toxic elements and thus prove as an effective method of water cleansing. Enzymatically active fungal BMOs can be harvested under specific cultivation conditions and remain active even under circumstances that would be unfavourable for fungal growth.[11]

Ginsenoside analogs

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Fermentation of ginsenoside Rb(1) with A. strictum yields three new compounds — 12β-hydroxydammar-3-one-20 (S)-O-β-D-glucopyranoside, 12β, 25-dihydroxydammar-(E)-20(22)-ene-3-O-β-D -glucopyranosyl-(1→2)-β-D -glucopyranoside, and 12β, 20 (R), 25-trihydroxydammar-3-O-β-D -glucopyranosyl-(1→2)-β-D -glucopyranoside — as well as five known compounds — ginsenoside Rd, gypenoside XVII, ginsenoside Rg, ginsenoside F, and compound K. Many of these compounds are metabolites of ginsenoside Rb(1) in mammals, suggesting that fermentation of ginsenoside Rb(1) in A. strictum may be similar to mammalian metabolism and may be a useful agent for generating specific metabolites or related ginsenoside analogs, which can be later isolated for structural elucidation and use in pharmaceutical research.[10]

References

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  1. ^ a b c d e f g h Guarro, J; Gams, W; Puhhol, I; Gene, J (1997). "Acremonium species: new emerging fungal opportunists—in vitro antifungal susceptibilities and review". Clinical Infectious Diseases. 25 (5): 1222–1229. doi:10.1086/516098. PMID 9402385.
  2. ^ a b c d e f Schell, WA; Perfect, JR (1996). "Fatal, disseminated Acremonium strictum infection in a neutropenic host". Journal of Clinical Microbiology. 34 (5): 1333–6. doi:10.1128/JCM.34.5.1333-1336.1996. PMC 229014. PMID 8727935.
  3. ^ a b c d e f g h i j k l m Sharma, Ajanta; Hazarika, N.K; Barua, Purnima; Shivaprakash, M.R.; Chakrabartie, Arunalok (2013). "Acremonium strictum: report of a rare emerging agent of cutaneous hyalohyphomycosis with review of literatures". Mycopathologia. 176 (5–6): 435–441. doi:10.1007/s11046-013-9709-1. PMID 24121988. S2CID 8507289.
  4. ^ a b c d e f Fincher, RM; Fisher, JF; Lovell, RD; Newman, CL; Espinel-Ingroff, A; Shadomy, HJ (1991). "Infection due to the fungus Acremonium (cephalosporium)". Medicine. 70 (6): 398–409. doi:10.1097/00005792-199111000-00005. PMID 1956281. S2CID 20440856.
  5. ^ a b c d e f g h i Perdomo, H.; Sutton, D.A.; Garcia, D.; Fothergill, A.W.; Cano, J.; Gene, J.; Summerbell, R.C.; Rinaldi, M.G.; Guarro, J. (2010). "Spectrum of clinically relevant Acremonium species in the United States". Journal of Clinical Microbiology. 49 (1): 243–256. doi:10.1128/jcm.00793-10. PMC 3020405. PMID 21068274.
  6. ^ a b c Rivera-Varas, VV; Freeman, TA; Gudmestad, NC; Secor, GA (2007). "Mycoparasitism of Helminthosporium solani by Acremonium strictum". Phytopathology. 97 (10): 1331–1337. doi:10.1094/phyto-97-10-1331. PMID 18943692.
  7. ^ a b c d Chalfoun, NR; Grellet-Bournonville, CF; Martinez-Zamora, MG; Diaz-Perales, A; Castagnaro, AP; Diaz-Ricci, JC (2013). "Purification and characterization of AsES protein: a subtilisin secreted by Acremonium strictum is a novel plant defense elicitor". Journal of Biological Chemistry. 288 (20): 14098–14113. doi:10.1074/jbc.m112.429423. PMC 3656267. PMID 23530047.
  8. ^ a b Yang, T; Ma, S; Dai, CC (2014). "Drought degree constrains the beneficial effects of a fungal endophyte on Atractylodes lancea". Journal of Applied Microbiology. 117 (5): 1435–1449. doi:10.1111/jam.12615. PMID 25080260.
  9. ^ a b Zhou, Sheng-Liang; Yan, Shu-Zhen; Liu, Qi-Sha; Chen, Shuang-Lin (2014). "Diversity of endophytic fungi associated with the foliar tissue of a hemi-parasitic plant Macrosolen cochinchinensis". Current Microbiology. 70 (1): 58–66. doi:10.1007/s00284-014-0680-y. PMID 25154477. S2CID 14419930.
  10. ^ a b Chen, G.T.; Yang, M; Song, Y; Zhang, J.Q.; Huang, H.L.; Wu, L.J.; Guo, D.A. (2008). "Microbial transformation of ginsenoside Rb1 by Acremonium strictum". Applied Microbiology and Biotechnology. 77 (6): 1345–1350. doi:10.1007/s00253-007-1258-4. PMID 18040682. S2CID 3262145.
  11. ^ a b Chang, Jianing; Tani, Yukinori; Naitou, Hirotaka; Seyama, Haruhiko (2013). "Fungal Mn oxides supporting Mn(II) oxidase activity as effective Mn(II) sequestering materials". Environmental Technology. 34 (19): 2781–2787. doi:10.1080/09593330.2013.790066. PMID 24527642. S2CID 42496840.
  12. ^ a b c d e f Summerbell, R.C.; Gueidan, C.; Schroers, H-J.; de Hoog, G.S.; Starink, M.; Arocha Rosete, Y.; Guarro, J.; James, J.A. (2011). "Acremonium phylogenetic overview and revision of Gliomastix, Sarocladium, and Trichothecium". Studies in Mycology. 68: 139–162. doi:10.3114/sim.2011.68.06. PMC 3065988. PMID 21523192.
  13. ^ Skrobot, F; Aglan, HA; Kitchens, S; Ludwick, A; Amburgey, T; Hamid, B; Diel, SV (2014). "Fungal populations in air and materials in a flood simulation study". Wood and Fiber Science. 46 (3): 2–15.
  14. ^ a b Leslie, J.F. (2008). Sorghum and Millets Diseases. John Wiley & Sons. pp. 188–189. ISBN 978-0470384701.
  15. ^ a b Goswami, Jaideep; Kumar Pandey, Rajesh; Tewari, J.P.; Goswami, B.K. (2008). "Management of root knot nematode of tomato through application of fungal antagonists, Acremonium strictum and Trichoderma harzianum". Journal of Environmental Science and Health. 43 (3): 237–240. Bibcode:2008JESHB..43..237G. doi:10.1080/03601230701771164. PMID 18368544. S2CID 25912156.
  16. ^ a b c Campbell, C.K; Johnson, E.M. (2013). Identification of Pathogenic Fungi. John Wiley & Sons. pp. 178–179. ISBN 978-1118520048.
  17. ^ a b Novicki, TJ; LaFe, K; Bui, L; Geise, R; Marr, K; Cookson, BT (2003). "Genetic diversity among clinical isolates of Acremonium strictum determined during an investigation of a fatal mycosis". Journal of Clinical Microbiology. 41 (6): 2623–8. doi:10.1128/jcm.41.6.2623-2628.2003. PMC 156529. PMID 12791889.
  18. ^ a b Guarro, J; Del Palacio, A; Cano, J; Gonzalez, CG (2009). "A case of colonization of a prosthetic mitrial valve by Acremonium strictum". Revista Iberoamericana de Micología. 26 (2): 146–148. doi:10.1016/s1130-1406(09)70025-7. PMID 19631164.
  19. ^ a b c Sener, AG; Yucesoy, M; Senturkun, Seckin; Afsar, Ilhan; Yurtsever, SG; Turk, M (2008). "A case of Acremonium strictum peritonitis". Med Mycol. 46 (5): 495–497. doi:10.1080/13693780701851729. PMID 18608934.
  20. ^ a b Koc, A; Sariguzel, M; Artis, T (2008). "Pleuritis caused by Acremonium strictum in a patient with colon adenocarcinoma". Mycoses. 51 (6): 554–556. doi:10.1111/j.1439-0507.2008.01517.x. PMID 18422911. S2CID 484712.
  21. ^ a b Racedo, J; Salazar, SM; Castagnaro, P; Diaz Ricci, JC (2013). "A strawberry disease caused by Acremonium strictum". European Journal of Plant Pathology. 137 (4): 649–654. doi:10.1007/s10658-013-0279-3. hdl:11336/7178. S2CID 18228356.
  22. ^ a b c Julianti, Elin; Oh, Hana; Jang, Kyoung Hwa; Lee, Jae Kyun; Lee, Sang Kook; Oh, Dong-Chang; Oh, Ki-Bong; Shin, Jongheon (2011). "Acremostrictin, a highly oxygenated metabolite from the marine fungus Acremonium strictum". Journal of Natural Products. 74 (12): 2592–2594. doi:10.1021/np200707y. PMID 22136576.
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